mouse tgf-beta 1 duoset elisa  (Bio-Techne corporation)

Journal: Theranostics

Article Title: Prevention of radiotherapy-induced pro-tumorigenic microenvironment by SFK inhibitors

doi: 10.7150/thno.100970

Figure Lengend Snippet: Radiotherapy induces macrophage infiltration and M2 differentiation. ( A, B ) Changes in immune cells infiltration in lung tissues. Lung tissues were collected 3 days after radiation. n = 6/group. ( C, D ) Changes in macrophage infiltration and M2 differentiation in lung tissues, 2 weeks after radiation (22 Gy×2). n = 5 or 6/group. The gating strategy and the percentages of M1 and M2 macrophages are shown in . ( E ) Fluorescence of vivotrack680-labeled monocyte and monocyte-derived macrophages. n = 7/group. ( F ) Infiltration and M2 differentiation of vivotrack680-labeled cells in lung tissues. ( G ) Identification of Tgf beta 1 mRNA levels in lung tissue after chest irradiation (22 Gy×2). Mice were sacrificed 2, 7, 14, and 28 days after irradiation. n = 5/group. C57BL6 mice were used. ( H ) Identification of secreted TGF beta 1 protein levels in lung tissue after chest irradiation. Mice were sacrificed 2 and 7 days after irradiation. n = 4/group. C57BL6 mice were used. ( I ) M2 differentiation of BMDMs 24 h after direct radiation exposure. ( J ) Western blot analyses for M1 and M2 markers in BMDMs 24 h after direct radiation exposure. ( K ) Measurement of Tgf beta 1 mRNA levels 24 h after direct irradiation of BMDM (12 Gy). ( L ) Measurement of TGF beta 1 protein secretion 24 h after direct irradiation of BMDM (12 Gy). ( M, N ) Schematic illustrations representing Figure results. All data were presented as mean ± SD. Statistical significance of the differences was determined by two-tailed Student t-test or one-way ANOVA followed by the Tukey's test. All western blot analyses in this study were repeated three times independently.

Article Snippet: Supernatants obtained from each experiment were measured using the DuoSet mouse TGF-beta 1 ELISA kit (#DY1679, R&D Systems, Minneapolis, MN, USA).

Techniques: Fluorescence, Labeling, Derivative Assay, Irradiation, Western Blot, Two Tailed Test

Journal: European Journal of Medical Research

Article Title: Berberine inhibits colorectal liver metastasis via modulation of TGF-β in a cecum transplant mouse model

doi: 10.1186/s40001-024-02122-w

Figure Lengend Snippet: Anti-metastatic effects of berberine in an orthotopic CRC mouse model. After the implantation of CT26 tumor tissue in the cecum (20 mg), berberine (100 mg/kg, from 3 to 21 days) was orally administered. Naked-eye photos ( A ) and histological analysis ( B ) of liver tissue are displayed. The number of tumors that metastasized to the liver was counted ( C ). The levels of four inflammatory cytokines (TGF-β, TNF-α, IL-6, and IL-1β) were determined using ELISA kits ( D–G ). The data are expressed as the means ± SD ( n = 5, n = 3). * p < 0.05 and ** p < 0.01 indicate statistical significance compared with the vehicle group

Article Snippet: The concentration level of inflammatory cytokines TGF-β (DY1679), TNF-α (DY410), IL-6 (DY406) and IL-1β (DY401) were determined in mouse serum using ELISA kits (R&D Systems).

Techniques: Enzyme-linked Immunosorbent Assay